Use of Adenoviral Vectors with a Minimal Cytomegalovirus Promoter
نویسندگان
چکیده
منابع مشابه
Use of adenoviral vectors with a minimal cytomegalovirus promoter.
E1-deleted recombinant adenoviruses 5 are widely used as gene vectors for basic and clinical research. Most adenoviral constructions bear an expression cassette inserted into the E1 region of their genome close to the 5′ end and include a heterologous promoter. The major immediate early human cytomegalovirus (hCMV) and the Rous sarcoma virus promoters, which have fairly high and consistent acti...
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BACKGROUND The full length Rad51 promoter is highly active in cancer cells but not in normal cells. We therefore set out to assess whether we could confer this tumor-selectivity to an adenovirus vector. METHODOLOGY/PRINCIPAL FINDINGS Expression of an adenovirally-vectored luciferase reporter gene from the Rad51 promoter was up to 50 fold higher in cancer cells than in normal cells. Further ev...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2003
ISSN: 0736-6205,1940-9818
DOI: 10.2144/03343dd02